Syngeneic tumor model and tumor measurement

Male and female WT and M-BKO mice aged 10–12 weeks were subcutaneously injected in the right flank with 300,000 B16-F10 mouse melanoma cells. For co-injection experiments, 500,000 B16-F10 cells were mixed with either 500,000 WT or M-BKO BMDMs (differentiation for 6 day) in the right and left flanks, respectively. Tumor dimensions were measured every two days by caliper after all mice had palpable tumors, and tumor volume was calculated as LxWxWx0.52 as previously described (Colegio et al., 2014). For DMM treatment, mice were switched to a soft pellet, high fat diet (Bio-Serv, F3282) so that DMM could be mixed with the diet using a blender. The tumor growth rate was slower on high fat diet (Figure 7C) compared to normal chow (Figure 7A).