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2.4. PC12 Cell Differentiation Analysis

MR Monique Rascher
KW Kathrin Wittstein
ZR Zeljka Rupcic
MS Marc Stadler
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PC12 differentiation: PC12 cells were analyzed by transmitted light (DM5500, Leica Microsystems, Wetzlar, Germany) or laser scanning confocal microscopy (SP8, Leica Microsystems) for neurite outgrowth. Cells containing neurites longer than one cell diameter in length were classified as differentiated. Three independent experiments were performed in doublets with 100 randomly picked cells analyzed for each well (n = 600 cells analyzed in total) to determine an average differentiation ratio.

Neurite length analysis: in addition, 10 neurites each from six independent randomly selected visual fields recorded by transmitted light microscopy obtained from three independent experiments (180 neurites in total) were measured to determine an average neurite length.

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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