MTT Assay

Plated SGC7901 and MGC803 cells were cultured in the presence or absence of germacrone (0, 50, 100, 150, 200, 250, and 300 μM) for 24, 48, and 72h. Proliferation was measured by MTT assay (Beyotime Biotechnology) to determine the effects of germacrone on cell proliferation. After germacrone treatment, MTT solution (5 mg/ml in PBS) was added (20 μl/well) and the plates incubated for another 4 h at 37°C. The formed purple formazan crystals were dissolved in 150 μl/well of DMSO. After 10 min, the absorbance at 490 nm was read using a microplate reader (Biotech Instruments, Dusseldorf, Germany).