2.16. LPS-Induced Peritonitis In Vivo Model

A protocol using C57BL/6 mice (10 mice/group) was followed to generate a peritonitis mouse model, as reported previously [26]. Rb-ME (50 and 100 mg/kg) that was suspended in 0.5% sodium carboxymethylcellulose (CMC) was orally administered once per day for five days. Acute peritonitis was evoked by an IP injection of 1.0 μL (10 μg/kg) LPS on day 4 after Rb-ME administration. The peritoneal fluid was harvested by peritoneal lavage using sterile PBS on day 5. NO production and the number of leukocytes in peritoneal exudates were measured by NO assay and counted while using a Neubauer chamber after staining with Turk solution.