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Hemolytic assay

LC Liangliang Chen
TS Tengfei Shen
YL Yongqing Liu
JZ Jiangfei Zhou
SS Shuaibing Shi
YW Yang Wang
ZZ Zhanqin Zhao
ZY Zhiling Yan
CL Chengshui Liao
CW Chen Wang
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The hemolytic activity of Chol-37(F34-R) was investigated as described previously [44]. Mouse erythrocytes were diluted to a final suspension concentration of 8% (v/v). Then, 100 μL erythrocyte suspension was incubated with 100 μL peptides at different concentrations ranging from 2.5 to 1280 μg/mL. PBS and 0.2% Triton X-100 were used as negative and positive controls, respectively. After 1 h of incubation, the tested samples were centrifuged for 5 min at 1000 g. Lastly, the absorbance of the supernatant of tested samples was measured at 570 nm. The percentage of hemolytic was obtained using the following formula: Hemolysis rate = [OD570nm (peptides) − OD570nm (PBS)]/[OD570nm (Triton X − 100) − OD570nm (PBS)] × 100 % .

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