Mice were injected with Dir-labelled nanoparticles, and PDT was delivered, as previously reported, for 15 min (20). At various points (3 h, 6 h, 10 h, 24 h) post-PDT, fluorescent scans (Ex/Em: 730/790 nm) were conducted on anesthetized mice using the Maestro 2 In Vivo Imaging System (CRI, USA). Mice were euthanized 24 h post-PDT, and tumors were harvested. Each tumor was washed with PBS and then subjected to fluorescence imaging.
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