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ALP staining was monitored using an ALP staining kit according to the manufacturer’s protocol. Mineral deposition was monitored using a Von Kossa staining kit according to the manufacturer’s protocol. Images were obtained with Nikon EclipseTi microscope (Nikon, Japan). Color intensity of mineral deposition was quantified by ImageJ.
Copyright and License information: ADMC Associazione Differenziamento e Morte Cellulare ©2018
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