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2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate (DCFH-DA) Staining
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After the indicated treatment, the production of ROS was measured by staining ESCs with the fluorescent dye DCFH-DA (Thermo Fisher Scientific, USA). Briefly, the cells were washed 3 times and loaded with 5 μM DCFH-DA, then incubated for 30 min at a temperature of 37°C in darkness. A confocal microscope (Zeiss, Germany) was used to quantify the resulting DCFH-DA fluorescence under 488 nm excitation light.
Copyright and License information: ©2020 Sun et al.
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