Bioluminescence and NIRF imaging for tumor xenograft models

Mice bearing xenograft tumors were injected intraperitoneally with MHI-148 at a dose of 50 nmol/mouse. Whole-body or organ-specific optical imaging was taken at 24 hr using a Caliper Lumina XR Imaging System (PerkinElmer, Waltham, MA) equipped with near-infrared fluorescent filter sets (excitation/emission, 783/840 nm) following a previously described protocol [8, 13]. Part of each piece of tumor tissue was processed as a frozen section and subjected to NIRF microscopy using a 75 W Xenon lamp and an indo-cyanine green filter cube (excitation/emission: 750-800/820-860 nm) as described above. Bioluminescence imaging of luciferase-tagged SGC-7901 tumor xenografts either in vivo or ex vivo after mice received D-luciferin (3 mg/mouse via intraperitoneal delivery) was performed on a Xenogen IVIS Spectrum Imaging System (PerkinElmer).