Detection of Y chromosome microdeletion and SRY

The detection was conducted in men with severe oligozoospermia or NOA. Eleven sequence-tagged sites (STS) from the AZF region were selected to explore the submicroscopic deletion. The STS markers were ZFY and SRY for internal control region. Samples from normal fertile male were used as positive control. Genomic DNA was extracted from peripheral blood lymphocytes according to practice guidelines. Multiplex polymerase chain reaction analysis was performed to investigate the deletion on SRY or AZF region by utilizing the testing system of YCMD. If STS deletions were detected, another independent test of the sample would be conducted repeatedly as described above.