DNA extraction, amplification, and sequencing

DNA was extracted from dried basidioma tissue of types and additional specimens using the Wizard^® Genomic DNA Purification kit (Promega Co., WI, USA) following manufacturer's instructions. For the type specimens, two DNA gene loci were sequenced from the nuclear ribosomal repeat, including the internal transcribed spacer (ITS) and the large subunit (nrLSU). The ITS was sequenced for most of the duplicates to corroborate conspecificity. Primer pairs ITS1F/ITS4B (Gardes & Bruns 1993) and LROR/LR6 (Vilgalys & Hester 1990, Moncalvo et al. 2000) were used to amplify ITS and nrLSU, respectively. Amplification reactions included 12.5 μL of Promega PCR Mastermix (Promega Co., WI, USA), 1.25 μL of each primer (at 10 μM) and approximately 100 ng of DNA. The final PCR reaction volume was 25 μL. The recommended cycling conditions for each primer pair were followed. PCR products were sequenced by GeneWiz^® (South Plainfield, NJ, USA). Sequences were edited using Sequencher v. 5.2.3 software (Gene Codes Corporation, MI, USA) and deposited in GenBank.