Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
DNA extraction from human dried blood spots (DBS)
Ask a
question
Favorite
DBS collected from 20 human subjects with known M. perstans infection and living in the Okola health district (Cameroon) were shipped to Montpellier to be used as references for phylogenetic analyses [3]. DNA extraction from DBS was performed using the NucliSENS® kit (bioMérieux, Craponne, France) according to the manufacturer’s instructions, as previously described [21]. DNA was eluted in 60 µl buffer. The PCR reactions on the M. perstans mf-positive human blood samples were carried on once all other samples had been tested.
Copyright and License information: The Author(s) ©2020
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
