Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Insulin tolerance test (ITT)

XX Xiaochun Xiong
JM John G. Menting
MD Maria M. Disotuar
NS Nicholas A. Smith
CD Carlie Delaine
GG Gabrielle Ghabash
RA Rahul Agrawal
XW Xiaomin Wang
XH Xiao He
SF Simon J. Fisher
CM Christopher A. MacRaild
RN Raymond S. Norton
JG Joanna Gajewiak
BF Briony E. Forbes
BS Brian J. Smith
HS Helena Safavi-Hemami
BO Baldomero Olivera
ML Michael C. Lawrence
DC Danny Hung-Chieh Chou
request Request a Protocol
ask Ask a question
Favorite

Insulin tolerance tests (ITT) was performed in 3–4 h fasted Sprague-Dawley rats (age: 12 weeks). Following baseline blood glucose measurements, rats were injected with either lispro (0.014 mg kg−1) or mini-Ins (0.014 mg kg−1) intraperitoneally. Tail vein samples were obtained to assess blood glucose levels every 15 min over 150 min using a glucometer (Ascensia Contour BG monitors, Bayer HealthCare, IN). Animal protocols were approved by local IACUC committee.

Copyright and License information:  ©2020
Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A