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Protein content of IgG1 was determined using UV spectrophotometer (make: Shimadzu, model: UV-1800) at 280 nm. The samples were analyzed by diluting to ~0.5 mg/mL, based on the concentration folds. (e.g., initial IgG1 volume of ~500 mL is reduced to ~250 mL, results in 2 fold concentration. Further concentration will result in reduction of retentate volume to 125 mL in which is 4 fold concentration, and so on).
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