Native agarose gel electrophoresis

Amplified DNA fragments and synthesized RNA were analyzed on an agarose gel using gel electrophoresis. To visualize the nucleotides, ethidium bromide (1:20,000) was added to the agarose gel after cooling it below 50°C. The separation of DNA fragments was performed with an agarose gel (1–3% agarose in 1xTAE), which was run with 90 V (70 ml gel) or 120 V (100 ml gel) in 1xTAE in Peqlab electrophoresis chambers. DNA and RNA fragments were visualized under UV light.