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The cells were transfected with gremlin siRNA or control siRNA (50 nM each). Transfections were performed using the riboFECT™ CP regeant according to the manufacturer' protocol. Assays were performed 48 h after transfection, including assessment of mRNA and protein levels and immunofluorescent staining.
Copyright and License information: ©2020 Qin et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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