Aβ40 and Aβ42 levels in the mouse hippocampus homogenates were determined with monoclonal and HRP-conjugated antibody-based Human/Rat β amyloid 40 ELISA kit (Wako, Osaka, Japan). Mouse TNF-α and IL-6 ELISA Ready-SET-Go! kits (Affymetrix, San Diego, CA, USA) were used for the detection of tumor necrosis factor α (TNF-α) and IL-6 in the conditioned media of WT and Akt2 KO mouse primary microglia cultures treated with LPS (200 ng/ml, Sigma-Aldrich, St. Louis, MO, USA) and IFNγ (20 ng/ml, Sigma-Aldrich, St. Louis, MO, USA) for 24 h. Nitric oxide (NO) levels were determined using the Griess Reagent Kit for Nitrite Determination (G-7921, Life Technologies, Eugene, OR, USA). All kits were used as recommended by the manufacturers.
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