Colistin susceptibility testing was performed by the two-fold broth microdilution reference method according to ISO 20776–1:2019 (ISO 20776-1:2019, 2019) using Sensititre EUVSEC plates (Trek Diagnostic Systems, United States) having a colistin range from 1 to 16 mg/L. Colistin resistant isolates where those having a minimal inhibitory concentrations (MIC) higher than 2 mg/mL (Spellerberg and Fedor, 2003).
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