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LXR reporter luciferase assay

WZ Wenchen Zhao
JF Jianjia Fan
IK Iva Kulic
CK Cheryl Koh
AC Amanda Clark
JM Johan Meuller
OE Ola Engkvist
SB Samantha Barichievy
CR Carina Raynoschek
RH Ryan Hicks
MM Marcello Maresca
QW Qi Wang
DB Dean G. Brown
AL Alvin Lok
CP Cameron Parro
JR Jerome Robert
HC Hsien-Ya Chou
AZ Andrea M. Zuhl
MW Michael W. Wood
NB Nicholas J. Brandon
CW Cheryl L. Wellington
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To interrogate LXR activation in CCF-STTG1 cells, the LXRα Cignal Reporter Assay kit (Qiagen) was used according to the manufacturer’s instructions. The LXRα reporter system is a mixture of a LXRα-responsive Firefly luciferase construct and a constitutively expressing Renilla luciferase construct (40:1) under the transcriptional control of a minimal CMV promoter and tandem repeats of LXR-response elements (LXRE). CCF-STTG1 cells were transfected with expression vectors containing WT or mutant LXRα without LXRE, followed by treatment with either 1 μM T0901317 or 3 μM AZ7235 24 h post transfection. LXRα activity was monitored by a dual luciferase assay (Promega) 24 h post treatment and luciferase activities were read on Tecan Spark Multimode Microplate Reader.

Copyright and License information: The Author(s) ©2020
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