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Cell viability assays were performed in 96-well plates. 2 × 103 143-B or 5 × 103 U2OS or SaOS-2 cells per well were seeded in tissue culture medium and allowed to adhere overnight. Increasing concentrations of cilengitide were added the next day and the cells were incubated for 6 or 24 hours. Following cilengitide treatment, the cells were incubated with 10 μl/well of WST-1 reagent (Roche, 05015944001) for 3 hours and the cell viability was then assessed as previously described [46]. Three independent experiments in triplicates were performed.
Copyright and License information: ©2016 Gvozdenovic et al.
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