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HPTLC instrumentation and chromatographic condition

PK Prabhjot Kaur
RG R. C. Gupta
AD Abhijit Dey
TM Tabarak Malik
DP Devendra Kumar Pandey
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HPTLC system (CAMAG) consists of 100 mL syringe (Hamilton, Bonaduz, Switzerland) and Linomat V automatic sample applicator (CAMAG, Muttenz, Switzerland). CAMAG glass twin trough chamber (20 cm × 10 cm × 4 cm) with stainless steel lid cover, CAMAG TLC Scanner III, CAMAG win Cats 3 integration software and UV cabinet with dual wavelength UV lamp (254 nm and 366 nm) was used for the HPTLC analysis. HPTLC analyses were performed on 20 × 10 cm pre-coated with silica gel 60 F254 (E-Merck) (0.2 mm thickness). HPTLC experiments were conducted at 25 ± 2 °C temperature and 40% relative humidity.

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