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ELISA assay of pro-inflammatory cytokines
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After LPS injection for 6 h, blood was collected from mice and centrifuged at 3,000 g for 10 min. The levels of IL-1β, IL-6, and TNF-α in the serum were measured using ELISA kits (Qiagen, Valencia, CA) according to the manufacturer’s instructions.
Copyright and License information: ©2016 by the American Society for Biochemistry and Molecular Biology, Inc.
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