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Analysis of PHGDH protein half-life.
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To determine the protein half-life of PHGDH in cells, cells were treated with CHX (50 μg/mL) or DMSO for indicated time periods (3–12 hours) before being collected for Western blot analysis of PHGDH protein levels using the anti-PHGDH antibody (catalog HPA021241, Sigma-Aldrich, 1:5000).
Copyright and License information: ©2020 American Society for Clinical Investigation
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