4.8. Measurement of iNOS Protein Expression and NO Production

RASMCs were pretreated with or without various inhibitors and then subjected to CMS for 4 h. The specific pretreatment details are described in the corresponding results section. Cell lysates were collected and assayed for iNOS protein expression by ELISA, according to the manufacturer’s instructions. The BioVision Nitric Oxide Colorimetric Assay Kit was used to determine NO production, according to the manufacturer’s instructions. NO is rapidly oxidized to nitrite and nitrate. Thus, these two metabolites in medium are used to quantitate NO production.