Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Quantitative PCR (qPCR) was performed to measure the density of Wolbachia in W-Aag-2 cells, as described previously (Tortosa et al., 2008). In brief, genomic DNA was extracted and wAlbB was amplified with the forward primer 183F (5'-AAGGAACCGAAGTTCATG-3') and the reverse primer QBrev2 (5'-AGTTGTGAGTAAAGTCCC-3'), which are specific for the Wolbachia surface protein (wsp) gene. The Wolbachia genome copy was normalized with the host rps6 gene.
Copyright and License information: ©2020 Lu, Sun, Fu, Li, Liang and Xi.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
