JAK2/STAT3 Pathway and NF-κB Pathway Measurement

Lentivirus encoding empty vector, encoding lnc-ITSN1-2 shRNA, and encoding lnc-ITSN1-2 shRNA/IL-23R overexpression were constructed by Shanghai Gene Company (Shanghai, China), and were transfected into IBD CD4⁺ T cells as the LV-scramble group, LV-anti-lnc-ITSN1-2 group, and LV-anti-lnc-ITSN1-2 & LV-IL-23R group. After 72 h transfection, JAK2, pJAK2, STAT3, pSTAT3, IKKA, and NF-κB expressions were detected by Western Blot assay according to the methods described in our previous study (16). The antibodies are listed in Supplementary Table 1.