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Molecular analysis of DNA

PD Pavel Dundr
MB Michaela Bártů
JH Jan Hojný
RM Romana Michálková
NH Nikola Hájková
IS Ivana Stružinská
EK Eva Krkavcová
LH Ladislav Hadravský
LK Lenka Kleissnerová
JK Jana Kopejsková
BH Bui Quang Hiep
KN Kristýna Němejcová
RJ Radek Jakša
OČ Otakar Čapoun
JŘ Jakub Řezáč
KJ Kateřina Jirsová
VF Věra Franková
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DNA analysis included mutation analysis of the coding parts of the HNF1B exons with adjacent intronic sequences (± 15 bp) and intronic regions containing the rs7527210 and rs4430796 polymorphisms and epigenetic analysis of CpG methylation in the region of the HNF1B promoter.

The HNF1B mutation analysis was performed using two different next-generation sequencing (NGS) approaches, depending on the type of tissue available. For 53 tumor samples and 49 corresponding non-tumor tissue for which the FT was available, the DNA samples were amplified by in-house 2-step polymerase chain reaction (PCR), and analyzed by amplicon next-generation sequencing (NGS). The 24 samples with only FFPE tissue available, which passed the DNA quality control criteria, were analyzed by a capture-based panel NGS, which is more suitable for FFPE samples and included all the coding parts of the HNF1B gene.

Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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