Urinary calcium and blood calcium were determined by methyl thymol blue colorimetric method, urinary phosphorus and blood phosphorus by reduction by ferrous ammonium sulphate hexahydrate and hydroxyproline (Hyp) by dimethylaminobenzaldehyde method. These kits were provided by Beijing Leagene Biotech. Co., Ltd, (Beijing, China). Alkaline phosphatase (ALP) was detected by aminoantipyrine colorimetric method, and the detection kit was provided by NanJing JianCheng Bioengineering Institute (Nanjing, China).
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