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4.13. Estimation of Th1/Th2 Cytokines Using ELISA

SK Sung-Dae Kim
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Th1/Th2 cytokines (i.e., IL-4 and IFN-γ) were measured using ELISA. 96 well plates were coated with 0.5 µg/mL CD3e mAb before incubation with splenocytes overnight. The MoAbs-based mouse interleukin ELISA kits (R&D system, Minneapolis, MN, USA) was used the following day to determine their levels, according to manufacturer’s instructions.

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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