4.4. Nitric Oxide (NO) Assay

Nitric oxide was measured by the method for the Griess reaction assay. RAW 264.7 cells were seeded in a 96-well plate and incubated with or without LPS (0.1 μg/mL) in the absence or presence of Rg3-RGE + PT at indicated concentrations for 18 h. Remaining steps were done as reported in our previous study [41]. Briefly, supernatant was collected and added with Griess reagent for the detection of NO. Absorbance of the resulting solution was checked at an absorbance of 540 nm using a microplate reader (Versamax; Molecular Devices, San Jose, CA, USA).