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Evans Blue Dye Leakage Assay

XY Xingyang Yi
CX Chongxi Xu
PH Pan Huang
LZ Linlei Zhang
TQ Ting Qing
JL Jie Li
CW Chun Wang
TZ Tao Zeng
JL Jing Lu
ZH Zhao Han
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The BBB permeability was evaluated using Evans blue extravasation (Zhang et al., 2018). Briefly, 2 h before sacrificed, the tail vein was injected intravenously 2% Evan’s blue dye at a dose of 4 ml/kg. Then, the rats were transcardially perfused with PBS and sacrificed. The concentration of Evan’s blue dye in the supernatant was assessed using spectrophotometer (610-nm wavelength). The results were showed as Evans blue (micrograms, μg)/brain tissue (g).

Copyright and License information:  ©2020 Yi, Xu, Huang, Zhang, Qing, Li, Wang, Zeng, Lu and Han
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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