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The subcellular localization of β-catenin was detected by PARIS ™ Kit (Ambion, Life Technologies, USA) according to the manufacturer's protocol. About 2*106 cells were collected and washed in cold PBS. After resuspending the cells in 500 μl ice-cold Cell Fractionation Buffer, the cytoplasmic fraction was aspirated away from the nuclear pellet which was lysed in Cell Disruption Buffer subsequently.
Copyright and License information: ©2016 Wang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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