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Genotyping and Quality Control

HL Hongxiang Lu
DW Dalin Wen
JS Jianhui Sun
JD Juan Du
LQ Liang Qiao
HZ Huacai Zhang
LZ Ling Zeng
LZ Lianyang Zhang
JJ Jianxin Jiang
AZ Anqiang Zhang
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Blood specimens were obtained from trauma patients when admitted to the hospital within 24 h. The genomic DNA was extracted from whole blood using a genomic DNA purification kit (Promega, Madison, WI, United States). Genotyping was performed using the SNPscan method in all samples following the manufacturer’s instructions (Li et al., 2015). One blank control in each plate was used for genotyping quality control, and 10% of samples were duplicated. The overall concordance rate was 100% among the duplicated samples. The genetic variants with a calling rate of >96%, minor allele frequency (MAF) of >0.01, and Hardy–Weinberg equilibrium (HWE) at P > 0.01 in the overall trauma cohort were included for further analysis. To calculate the wGRS, only the patients that completely genotyped for all genetic variants were included for investigation.

Copyright and License information:  ©2020 Lu, Wen, Sun, Du, Qiao, Zhang, Zeng, Zhang, Jiang and Zhang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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