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Desiccation tolerance assay

QR Qun Ren
RB Rebecca Brenner
TB Thomas C. Boothby
ZZ Zhaojie Zhang
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Cells were grown in 15 ml round-bottom glass tubes (Fisher Scientific, Waltham, MA) to log- or stationary phase in YPD medium. Cells were centrifuged (× 1000 g for 5 min) and the culture medium was discarded. The culture tubes without caps were placed in a humid chamber (23 °C, 50% relative humility) and cells were allowed to desiccate for desired days. After desiccation, cells were resuspended in water, vortexed for 30 s, then diluted to 3000 cells/ml. 100 μl of cells were plated on YPD agar plates (1% yeast extract, 2% peptone, 2% dextrose and 2% agar) for 2–3 days, and the number of colonies were counted. Non-desiccated cells at the same growth conditions were used as controls. 3–4 plates were used for each strain or growth condition. Desiccation tolerance (survival rate)(%) was calculated as (number of colonies of desiccated cells)/(number of colonies of non-desiccated cells) X 100 from 3 independent experiments.

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