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Mice were daily intrathecally injected with saline or morphine (10 μg/10 μl) for 7 consecutive days. Spinal cord segments were collected at L1–L6. The malondialdehyde (MDA) of mouse spinal cord samples was measured according to a previously described method (Gong et al., 2012). Briefly, the tissue samples subjected to MDA measurement were assayed with kits from Nanjing Jiancheng Bioengineering Institute according to manufacturer’s instructions. The products of reactions were detected by Multi-Mode Microplate Readers (Thermo Multiskan FC) at 532 nm.
Copyright and License information: ©2019 The Author(s) (2019). Published by Oxford University Press on behalf of , IBCB, SIBS, CAS.
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