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As an external measure of puberty onset in males, mice were restrained and gently examined daily from P27 to P33 (10:00‐12:00 am) on the potential to fully retract the prepuce and expose the glans penis which was designated as puberty onset. 35 Female mice were scored daily from P24 to P36 for vaginal opening, here taken as sign of puberty onset. 36 All mice were weighed at puberty onset.
Copyright and License information: ©2020 The Authors. Genes, Brain and Behavior published by International Behavioural and Neural Genetics Society and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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