Analysis of body composition and metabolic parameters in aging mice

Tobias Eisenberg; Mahmoud Abdellatif; Sabrina Schroeder; Uwe Primessnig; Slaven Stekovic; Tobias Pendl; Alexandra Harger; Julia Schipke; Andreas Zimmermann; Albrecht Schmidt; Mingming Tong; Christoph Ruckenstuhl; Christopher Dammbrueck; Angelina S. Gross; Viktoria Herbst; Christoph Magnes; Gert Trausinger; Sophie Narath; Andreas Meinitzer; Zehan Hu; Alexander Kirsch; Kathrin Eller; Didac-Carmona Gutierrez; Sabrina Büttner; Federico Pietrocola; Oskar Knittelfelder; Emilie Schrepfer; Patrick Rockenfeller; Corinna Simonini; Alexandros Rahn; Marion Horsch; Kristin Moreth; Johannes Beckers; Helmut Fuchs; Valerie Gailus-Durner; Frauke Neff; Dirk Janik; Birgit Rathkolb; Jan Rozman; Martin Hrabe de Angelis; Tarek Moustafa; Guenter Haemmerle; Manuel Mayr; Peter Willeit; Marion von Frieling-Salewsky; Burkert Pieske; Luca Scorrano; Thomas Pieber; Raimund Pechlaner; Johann Willeit

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Analysis of body composition and metabolic parameters in aging mice

TE Tobias Eisenberg

MA Mahmoud Abdellatif

SS Sabrina Schroeder

UP Uwe Primessnig

SS Slaven Stekovic

TP Tobias Pendl

AH Alexandra Harger

JS Julia Schipke

AZ Andreas Zimmermann

AS Albrecht Schmidt

MT Mingming Tong

CR Christoph Ruckenstuhl

CD Christopher Dammbrueck

AG Angelina S. Gross

VH Viktoria Herbst

CM Christoph Magnes

GT Gert Trausinger

SN Sophie Narath

AM Andreas Meinitzer

ZH Zehan Hu

AK Alexander Kirsch

KE Kathrin Eller

DG Didac-Carmona Gutierrez

SB Sabrina Büttner

FP Federico Pietrocola

OK Oskar Knittelfelder

ES Emilie Schrepfer

PR Patrick Rockenfeller

CS Corinna Simonini

AR Alexandros Rahn

MH Marion Horsch

KM Kristin Moreth

JB Johannes Beckers

HF Helmut Fuchs

VG Valerie Gailus-Durner

FN Frauke Neff

DJ Dirk Janik

BR Birgit Rathkolb

JR Jan Rozman

MA Martin Hrabe de Angelis

TM Tarek Moustafa

GH Guenter Haemmerle

MM Manuel Mayr

PW Peter Willeit

MF Marion von Frieling-Salewsky

BP Burkert Pieske

LS Luca Scorrano

TP Thomas Pieber

RP Raimund Pechlaner

JW Johann Willeit

This method is extracted from research article: Nat Med, Nov 2016

Cardioprotection and lifespan extension by the natural polyamine spermidine

DOI: 10.1038/nm.4222

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Body composition (relative lean- and fat mass) was determined by in vivo NMR spectroscopy using the Minispec mq NMR analyser (Brucker Optics, USA), according to the manufacturer’s instructions. General activity (assessed by the total number of beam breaks, XT+YT counts) as well as the respiratory exchange rate (RER, expressed as the volumetric quotient of carbon dioxide elimination of the animal divided by oxygen consumption, V_CO2/V_O2) was determined using PhenoMaster™ cages (TSE-Systems). Mice were singly housed and the first complete 12 hour dark- and light-cycles were recorded for parameter analysis after an initial adaptation phase of 8 hours.

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