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The concentration of cell-free double stranded DNA in human serum samples was quantitated with the Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher Scientific, Grand Island, NY, USA). Serum samples were diluted 10 to 100–fold in sterile PBS and PicoGreen reagent was added according to manufacturer’s instructions. DNA doses were quantitated using a known DNA standard and are expressed as ng/ml in undiluted human serum.
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