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Determination of the critical micelle concentration
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Ring tensiometry measurements were used to determine the critical micelle concentration (CMC) of compound 3. Samples with varying concentrations of 3 in a 0.5 M TRIS buffer (pH 8.00) were prepared and their surface tension was determined at 25 °C (in triplicate) using a Kruss K10ST tensiometer. The CMC (0.011 mM) is the point at which an increase in the concentration of 3 no longer results in a decrease in the surface tension (Supplemental Fig. 5).
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