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Generation of TDP-43O-specific polyclonal antibodies.

MM Mauro Montalbano
SM Salome McAllen
FC Filippa Lo Cascio
US Urmi Sengupta
SG Stephanie Garcia
NB Nemil Bhatt
AE Anna Ellsworth
EH Eric A. Heidelman
OJ Omar D. Johnson
SD Samantha Doskocil
RK Rakez Kayed
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TDP-43Os mixed with Freund’s complete adjuvant were used as antigen to immunize two New Zealand white rabbits (Pacific Immunology Corp. Ramona, CA). The immunization process was carried out according to the protocol approved by Pacific Immunology, where each rabbit was given a dose of 200 μg of antigen as the first injection. This was followed by three booster doses of 100 μg each. Sera from the immunized rabbits were tested in biochemical assays. The sera with the highest antigen binding and more specificity were affinity-purified in a protein A column and used for further analyses.

Copyright and License information:  ©2020-10-14

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