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Hoechst 33258 staining and apoptosis analysis by flow cytometry
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As we have described previously [9], the DS2-induced apoptosis was examined and identified according to the condensation and fragmentation of their nuclei by fluorescence microscopy after Hoechst 33258 staining. FITC- annexin V and PI was used to stain and identify subpopulations of cells with membrane changes (early stage apoptosis) and the associated loss of membrane integrity (necrotic or late apoptotic cells), respectively.
Copyright and License information: ©2016 Ma et al.
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