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We purchased 5-azacytidine (5-Aza) from Sigma-Aldrich (St. Louis, MO, USA). After cells were cultured to sub-confluence, 5-Aza was supplemented in the cultured at 10 μM. Cells were harvested after 24 h, and total RNA was extracted as described above.
Copyright and License information: ©2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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