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Measurement of dopamine levels by high-performance liquid chromatography (HPLC)

JL Jia Liu
MC Min Chen
XW Xue Wang
YW Yi Wang
CD Chunli Duan
GG Ge Gao
LL Lingling Lu
XW Xia Wu
XW Xiaomin Wang
HY Hui Yang
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Striatum tissue was weighed and homogenized in 0.1mol/l HClO4 and incubated on ice for 1h, then centrifuged at 12,000rpm at 4°C for 20 min. The supernatant was mixed with the HPLC mobile phase consisting of 63.5mM citric acid monohydrate, 60.9mM trisodium citrate dehydrate, 0.1M EDTA, and 0.5M C-8alkyl sulfonate (pH 4.3) and injected into the HPLC column under analytical conditions. Data are expressed as pg dopamine per mg tissue.

Copyright and License information:  ©2016 Liu et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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