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dSTORM

EB Emily I. Bartle
TR Tejeshwar C. Rao
RB Reena R. Beggs
WD William F. Dean
TU Tara M. Urner
AK Andrew P. Kowalczyk
AM Alexa L. Mattheyses
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dSTORM experiments were conducted on a Nikon Ti-2 N-STORM microscope equipped with a 100× 1.49-NA oil-immersion objective, 488- and 647-nm lasers, and an iXon ultra electron-multiplying charge-coupled device camera (Andor). 60,000-100,000 frames were collected with subcritical inclined excitation and reconstructed in Nikon Elements. Photoswitching imaging buffer included glucose oxidase (Sigma-Aldrich), glucose (Sigma-Aldrich), catalase (Roche), and β-mercaptoethanol (Sigma-Aldrich; Rust et al., 2006).

Copyright and License information:  ©2020 Bartle et al.
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