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Suspension of CaOx-F(F)F and Phos-MecycC5
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The suspension of CaOx-F(F)F (17.3 mM) and Phos-MecycC5 (2.4 mM) with/without NP-Alexa647 (4.0 µM) and NBD-cycC6 (4.0 µM) in 100 mM MES, pH 6.0 was heated by a heating gun until dissolving. The resultant mixture was cooled to rt and incubated at rt for 1 h. The state (gel or sol) of the sample was judged by the tube inversion test. The obtained suspension (containing fluorescent probes) was moved to a glass bottom dish and observed by CLSM imaging.
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