Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Feeder-independent E14 and R1 mES cells were cultured in 2i medium [1], a serum-free N2B27 medium supplemented with MEK inhibitor PD0325901 (1 μM) and GSK3 inhibitor CHIR99021 (3 μM) (both from Selleckchem), and 1000 U/ml LIF (Millipore). TrypLE™ Express (Life Technologies) was used to passage the cells. To ensure good adherence 2 % ES-qualified FBS was supplemented to the 2i media when seeding the cells, as previously described [2].
Copyright and License information: The Author(s) ©2016
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
