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RNA Extraction and Quantitative RT-qPCR Analysis
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Total RNA from 2-week-old plants was extracted with the RNeasy Plant Mini Kit (Qiagen) and treated with RNase-free DNase (Sigma-Aldrich). cDNA was synthesized by SuperScript II reverse transcriptase (Invitrogen). RT-qPCR was performed using the SYBR Green PCR Master Mix Kit (Bio-Rad). The gene-specific primer sequences are provided in Supplemental Table S1. The relative expression levels were analyzed by the comparative cycle threshold (ΔΔCt) method.
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