GSEA Analysis Across TCGA Pan-Cancer

To determine the effect of ACE2 expression on tumors, we divided the samples into high and low expression groups according to their gene expression level and used Gene Set Enrichment Analysis (GSEA) (Barber et al., 2006) to analyze the enrichment of KEGG and HALLMARK pathways in the high and low expression groups. The gene sets using the c5 curated signatures were downloaded from the Molecular Signature Database (MSigDB) of the Broad Institute. The KEGG terms were defined between the high ACE2 expression group and the low ACE2 expression group. The significant enrichment of pathways was determined based on FDR < 0.05.