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Cell viability was assessed as previously described with minor modifications15. Briefly, cultures were treated as described above. At DIV7 the experimental media were replaced with incubation medium and the number of viable cells was assessed by the Presto Blue assay (Invitrogen, Reinach, Switzerland) using a microplate reader (Ex. 560 nm, Em. 590 nm; VarioSkan, ThermoFisher Scientific, Reinach, Switzerland).
Copyright and License information: The Author(s) ©2020
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work is attributed as specified on the SAGE and Open Access
pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
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