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Total RNA was extracted from ~10 female mosquitoes of each group using TRIzol (Invitrogen, Carlsbad, CA, USA) as per the manufacturer’s protocol, after pulverizing the tissues in a homogeniser on ice. Total RNA was finally dissolved in 40 µl RNase-free water and 1 µg total RNA was reverse transcribed to cDNA in accordance with the instructions of the reverse transcription kit (Takara, Dalian, Liaoning, China).
Copyright and License information: The Author(s) ©2020
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